1. The dynamic relationship between aneuploidy and CIN Aneuploidy generates CIN, including increased chromosome loss, mutation rate and defective DNA damage repair [39, 119]. The relationship between aneuploidy and CIN can be envisioned as a “vicious cycle,” wherein one potentiates the other [120]. The “stress–CIN–cancer evolution relationship” can also be used to discuss the relationship between aneuploidy and cancer [50]. Elevated transcriptome dynamics are linked to karyotype changes which impact multiple genetic/epigenetic interactions [121,122,123] Aneuploidy is less influential compared to structure alterations [54]. CIN rates might be more predictive for tumor outcome than assessing aneuploidy rates alone [54, 124]. Many cancer cell lines with aneuploidy are relatively stable (an example of fuzzy inheritance of some relatively stable systems) [37]. Genome chaos, including karyoplast budding, giant cells and mitotic catastrophe, is often associated with aneuploidy [67, 125,126,127]. Chromosomal condensation defects (DMFs) and Chromosome fragmentation (C-Frags) can generate aneuploidy [37, 106, 118]. Aneuploidy (in the form of mosaicism) represents a common phenomenon. We may all have a touch of Down syndrome [128, 129]. Aneuploidy is a main feature among individual cancer cell lines. The rate of aneuploidy seems inherited [72]. Genomic PTEN deletion size influences the landscape of aneuploidy and outcome in prostate cancer [130]. ATM and p21 cooperate to suppress aneuploidy and tumor development [117] | |
2. The complex relationship between aneuploidy and immune response When co-cultured with natural killer cells, aneuploidy cells with complex karyotype-induced senescent cells were selectively cleared [131]. High copy number alterations in melanoma patients are linked with less effective response to immune checkpoint blockade anti–CTLA-4 [52]. | |
3. Biological impact of aneuploidy Aneuploidy changes the genomic coding, which affects the transcriptome, proteome, network structure, incidence of CIN and phenotypes [4, 37, 132]. Chromosome mis-segregation per se can alter the genome in many ways in addition to chromosome gain or loss [133]. Aneuploidy puts pressure on the protein machinery and quality control, which generates a global stress response, reducing cell proliferation [133]. Both specific gene effects and the typical aneuploidy stress response contribute to new genomic coding or/and increased system stress, which can impact the emergent process of cancer evolution ([133], current paper) Karyotype status (e.g. aneuploidy and polyploidy) can restore functions of specific genes (e.g. MYO1). Thus, genomic coding changes gene coding [83]. The chromosomal size involved in aneuploidy is inversely correlated to the resulting fitness [134]. The risk of cancers to metastasize is proportional to the degree of cancer-specific aneuploidy [48]. There is a dynamic relationship between epigenetic events and aneuploidy; epigenetic marks play a role in the control of chromosome segregation and integrity; aneuploidy impacts chromatin silencing [135,136,137]. New approaches are needed to study the complexity of systems, including that of aneuploidy-mediated karyotype evolution [138, 94, 110]. |